设为首页
加入收藏
联系我们
Email-Alert
 

    首页 | 杂志介绍 | 编委成员 | 审稿专家名录 | 投稿指南 | 订阅指南 | 过刊浏览 | 论著模板 | 综述模板 | 帮助

 
刘 乐,张学敏,崔珍珍,段博阳,王 月,王璐璐,唐久来,吴 德.人巨细胞病毒孕早期先天性感染模型子代大鼠孤独症谱系障碍症状研究[J].中国康复医学杂志,2020,(5):517~521
人巨细胞病毒孕早期先天性感染模型子代大鼠孤独症谱系障碍症状研究    点此下载全文
刘 乐  张学敏  崔珍珍  段博阳  王 月  王璐璐  唐久来  吴 德
安徽医科大学第一附属医院儿科小儿神经康复中心,安徽省合肥市,230022
基金项目:国家自然科学基金资助项目(81472167)
DOI:10.3969/j.issn.1001-1242.2020.05.002
摘要点击次数: 130
全文下载次数: 52
摘要:
      摘要 目的:建立人巨细胞病毒(HCMV)孕早期宫内感染模型,探讨子代大鼠ASD症状。探讨HCMV孕早期先天性感染致子代孤独症谱系障碍(ASD)症状。 方法:随机选取孕3天SD大鼠14只,实验组7只腹腔注射1×10-6TCID50病毒悬液0.5ml, 对照组7只腹腔注射人胚肺成纤维细胞(HELF)上清液0.5ml。仔鼠中随机各取6只,实验组母鼠的仔鼠记为HCMV组(n=6), 对照组母鼠的仔鼠记为HELF组(n=6),分别进行病理及行为学试验。 结果:病理及电镜结果显示实验组子代大鼠海马髓鞘破坏。Morris游泳距离:HCMV组1—4天总路程(d1:17.97±3.82;d2:15.22±2.96;d3:14.22±2.08;d4:10.87±2.34)m,高于HELF组1—4天总路程(d1:9.36±1.95;d2:6.31±1.65;d3:5.36±1.23;d4:5.01±1.24)m,差异有显著性意义(P<0.01)。刻板行为结果:HCMV组刻板动作次数(40.7±3.98,38.7±3.4,38.3±4.13,40.2±3.4,39.7±2.01,38.5±3.00)次,明显多于HELF组(18.5±2.70,21.7±3.17,26.4±3.26,20.2±2.29,19.2±2.94,20.9±3.00)次,差异有显著性意义(P<0.01)。旷场实验结果:HCMV组站立次数(19.15±3.63)次、跨格子数(41.70±11.05)均显著低于HELF组站立次数(22.05±4.14)次、跨格子数(66.05±12.57)(P<0.05)。悬吊试验HCMV组仔鼠得分(1.52±0.61)显著低于对照组(2.70±0.73)(P<0.01),斜坡试验HCMV组仔鼠评分(1.96±0.29)显著低于HELF组(4.25±0.48)(P<0.01)、倾斜板试验HCMV组停留时间(48.17±7.15)s显著低于HELF组(63.99±5.76)s(P<0.01)。 结论:HCMV孕早期感染,子代大鼠有ASD样症状,表明HCMV孕早期宫内感染可能是诱发ASD的病因之一。
关键词:人巨细胞病毒  孤独症谱系障碍  动物模型  神经行为学
Offspring symptoms of autism spectrum disorder in the rat’s SD model of HCMV congenital infection    Download Fulltext
Department of Children Rehabilitation, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022
Fund Project:
Abstract:
      Abstract Objective: The intrauterine infection model of HCMV in early pregnancy was established to investigate the ASD symptoms of offspring rats. Method: Fourteen SD rats with 3 days pregnant were randomly selected:7 rats in the experimental group were intraperitoneally injected with 0.5ml of 1×10-6TCID50 virus suspension,and 7 rats in the control group were intraperitoneally injected with 0.5 ml supernatant of human embryonic lung fibroblast (HELF). Six offspring rats were randomly selected from each group. The offspring rats of the experimental group were recorded as the HCMV group (n=6), and the offspring rats of the control group were recorded as the HELF group (n=6). Pathological and behavioral tests were conducted respectively. Result: Pathological and electron microscopy results showed that the hippocampal myelin sheath of the offspring of the experimental group was destroyed. Morris swimming distance: 1—4 days total distance of HCMV group (d1:17.97±3.82; d2:15.22±2.96; d3:14.22±2.08; d4:10.87±2.34)m,was higher than that of HELF group (d1:9.36±1.95; d2:6.31±1.65; d3:5.36±1.23; d4:5.01±1.24)m, and the difference was significant (P<0.01). Stereotyped behavior results:the number of stereotyped actions in the HCMV group (40.7±3.98, 38.7±3.4, 38.3±4.13, 40.2±3.4, 39.7±2.01, 38.5±3.00) times was significantly more than the HELF group (18.5±2.70, 21.7±3.17, 26.4±3.26, 20.2±2.29, 19.2±2.94, 20.9±3.00) times,and the difference was significant (P<0.01). Open field experiment results:the number of standing times (19.15±3.63) and the number of cross lattices (41.70±11.05) in the HCMV group were significantly lower than the number of standing times (22.05±4.14) and the number of cross lattices (66.05±12.57) in the HELF group (P<0.05). The suspension test:The score of offspring in the HCMV group (1.52±0.61) was significantly lower than that in the control group (2.70±0.73) (P<0.01),and the score of the slope test in the HCMV group (1.96±0.29) was significantly lower than that in the HELF group (4.25±0.48) (P<0.01). In the inclined plate test, the residence time of HCMV group (48.17±7.15)s was significantly lower than HELF group (63.99±5.76)s (P<0.01). Conclusion: Early infection of HCMV pregnancy and asd-like symptoms in offspring rats suggest that early intrauterine infection of HCMV pregnancy may be one of the causes of ASD induction.
Keywords:human cytomegalovirus  autism spectrum disorders  animal models  neuroethology
查看全文  查看/发表评论  下载PDF阅读器

您是本站第 26765912 位访问者

版权所有:中国康复医学会
主管单位:国家卫生健康委员会 主办单位:中国康复医学会
地址:北京市朝阳区樱花园东街,中日友好医院内   邮政编码:100029   电话:010-64218095   

本系统由北京勤云科技发展有限公司设计 京ICP备18060696号-2