设为首页
加入收藏
联系我们
Email-Alert
 

    首页 | 杂志介绍 | 编委成员 | 审稿专家名录 | 投稿指南 | 订阅指南 | 过刊浏览 | 论著模板 | 综述模板 | 帮助

 
苏美华,刘晓莉,乔德才.运动疲劳对大鼠皮质M1区mGluR1和mGluR5蛋白表达的影响[J].中国康复医学杂志,2020,(1):5~9
运动疲劳对大鼠皮质M1区mGluR1和mGluR5蛋白表达的影响    点此下载全文
苏美华  刘晓莉  乔德才
北京师范大学体育与运动学院,北京,100875
基金项目:福建省自然科学基金面上项目(2019J01754);福建省高校杰出青年人才计划项目(2005-B11504)
DOI:10.3969/j.issn.1001-1242.2020.01.002
摘要点击次数: 59
全文下载次数: 48
摘要:
      摘要 目的:疲劳是导致机体工作能力下降的重要原因,通过观察反复力竭运动大鼠皮质主运动区中mGluR1和mGluR5在运动前后的表达变化,探讨Ⅰ组代谢型谷氨酸受体介导运动疲劳产生的作用效应。 方法:选用雄性Wistar大鼠32只,随机分为对照组(CG)、力竭即刻组(0EG)和运动后24h组(24EG),运动后48h组(48EG),每组8只,采用免疫组化染色和计算机图像分析系统检测并观察各组mGluR1和mGluR5表达的阳性细胞数和积分光密度(IOD)。 结果:与对照组相比,力竭后即刻组及运动后24h组,大鼠皮质主运动区中mGluR1和mGluR5阳性细胞数量和IOD值均显著升高(P<0.05);运动后48h组mGluR1的蛋白表达较对照组仍有显著升高(P<0.05),而运动后48h组的mGluR5蛋白表达和对照组无显著差异(P>0.05)。 结论:运动疲劳可导致大鼠中枢运动皮质代谢型谷氨酸受体的表达上调,不同亚型受体蛋白表达的时间效应不一样,提示mGluR1和mGluR5可能是中枢神经系统中介导运动疲劳产生的重要新型受体靶点。
关键词:运动疲劳  大鼠  皮质  代谢型谷氨酸受体  M1区
Effects of exercise fatigue on mGluR1 and mGluR5 expression in primary motor cortex of rats    Download Fulltext
Beijing Normal University, 100875 and Beijing Normal University, 100875
Fund Project:
Abstract:
      Abstract Objective: Fatigue is the crucial reason for the decline of working ability. By observing the expression changes of mGluR1 and mGluR5 in cortex M1 zone of rats pre and post exercise, the paper discussed the effects of mGluR1 and mGluR5 on the central nervous system induced by the exhaustive exercise. Method: Totally 32 male Wistar rats were randomly divided into four groups,CG, 0EG, 24EG and 48 EG, 8 rats for each. Meanwhile, immunohistochemistry (IMM) technique was used to investigate the expression of positive cell and integrated optical density (IOD) of mGluR1 and mGluR5 in different groups. Result: Compared with CG, the expression of positive cell and integrated optical density (IOD) of mGluR1 in 0EG and 24EG both significantly increased (P<0.01). The expression of mGluR5 in 0EG and 24EG (P<0.01) also enhanced dramatically compared with CG. However, the expression of mGluR1in the 48EG was still higher than the CG(P<0.05), while the expression of mGluR5 in the 48EG was not different from CG (P>0.05). Conclusion: Repeated exhaustive exercise could result in up-regulation the expression of mGluR1 and mGluR5 in cortex M1 zone of rats, which suggested that mGluR1 and mGluR5 with different time effects were another important receptors related with the production of exercise fatigue.
Keywords:exercise fatigue  rats  cortex  mGluRs  M1 zone
查看全文  查看/发表评论  下载PDF阅读器

您是本站第 24392928 位访问者

版权所有:中国康复医学会
主管单位:国家卫生健康委员会 主办单位:中国康复医学会
地址:北京市朝阳区樱花园东街,中日友好医院内   邮政编码:100029   电话:010-64218095   

本系统由北京勤云科技发展有限公司设计 京ICP备18060696号-2