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江一静,林志诚,游咏梅,王 君,杨珊莉.巨刺法对脑缺血再灌注损伤大鼠运动功能、脑影像结构及PI3K/AKT信号通路的影响[J].中国康复医学杂志,2019,(9):1014~1020
巨刺法对脑缺血再灌注损伤大鼠运动功能、脑影像结构及PI3K/AKT信号通路的影响    点此下载全文
江一静  林志诚  游咏梅  王 君  杨珊莉
福建中医药大学附属康复医院,福州,350003
基金项目:国家自然科学基金项目(81574048);福建省科技厅平台建设项目(2015Y2001);高校青年自然基金重点项目(JZ160443)
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摘要:
      摘要 目的:观察巨刺法对脑缺血再灌注损伤模型大鼠运动功能及影像学结构的影响,并探讨其可能的作用机制。 方法:将30例健康雄性SD大鼠随机分为假手术组、模型组及巨刺组,各10只。模型组及巨刺组大鼠接受改良局灶性脑缺血再灌注大脑中动脉栓塞(MCAO)模型制备,假手术组仅接受切开皮肤+分离血管+皮肤缝合处理。巨刺组进行巨刺曲池穴及足三里穴7d,模型组及假手术组仅接受模拟捉拿及相应穴位点触。各组采用神经行为学评分评估大鼠运动功能,H反射评估大鼠肌张力状况,TTC染色及MRI观察脑梗死体积,Western Blot检测PI3K、AKT、p-AKT水平,免疫组化检测Caspase-3的表达,Tunel检测神经元细胞调亡情况。 结果:巨刺法可明显降低MCAO大鼠的神经行为学评分、H反射的潜伏期、脑梗死体积、神经元凋亡率以及Caspase-3表达,增加H反射的波幅、PI3K、p-AKT表达水平。 结论:巨刺法可明显改善脑缺血再灌注损伤大鼠的运动功能,其作用机制可能与介导PI3K/AKT信号通路有关。
关键词:脑缺血再灌注损伤  运动功能  巨刺法  H反射  PI3K/AKT信号通路
The impact of opposing needling on motor function, the structure of the brain images and PI3K/AKT signaling in MCAO rats    Download Fulltext
Rehabilitation Hospital Affiliated to Fujiang University of Traditional Chinese Medicine, Fuzhou, 350003
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Abstract:
      Abstract Objective: To observe the effect of opposing needling on movement function and the imaging structure in the cerebral ischemia-reperfusion injury model rats, and to discuss the possible mechanism of action. Method: Thirty cases of healthy male Sprague Dawley(SD) rats were randomly divided into the control group, the model group and the opposing needling group, 10 in each group. The rats in model group and opposing needling group accepted the modified middle cerebral artery occlusion (MCAO Preparation) model, the control group only accepted skin incision +blood vessels separation + skin suture. The opposing needling group accepted opposing needling at acupoints of “LI 11”and “ST 36” for 7 d, the model group and control group only accepted analog capture. In each group, the movement function in rats were evaluated by neurobehavioral scores, reviewed the status of rat muscle tone by H reflex, observed the cerebral infarction volume by TTC staining and MRI, tested PI3K, AKT, p-AKT by Western Blot. The expression of Caspase 3.Tunel was used to detect the apoptosis of neuron cells. Result: The opposing needling obviously reduced the neurobehavioral scores, H reflex of the incubation period, brain infarct volume,apoptosis rate of neurons and the expression of Caspase 3 in MCAO rats , increased the amplitude of H reflection, the expression levels of PI3K and p-AKT. Conclusion: opposing needling can obviously improve the motor function in cerebral ischemia reperfusion injury rats, the mechanism may be related to the mediation of PI3K/AKT signal pathway.
Keywords:cerebral ischemia-reperfusion injury  motor function  opposing needling  H reflection  PI3K/AKT signaling pathway
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