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彭 勇,史绍蓉,黄思敏,徐 哲,郭远盘.基于蛋白质组学的中等强度有氧运动对大鼠心房肌物质能量代谢蛋白差异表达的研究[J].中国康复医学杂志,2019,(5):521~526
基于蛋白质组学的中等强度有氧运动对大鼠心房肌物质能量代谢蛋白差异表达的研究    点此下载全文
彭 勇  史绍蓉  黄思敏  徐 哲  郭远盘
湖南师范大学体育学院
基金项目:湖南省科技厅计划项目(2007FJ3075)
DOI:
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摘要:
      摘要 目的:基于蛋白质组学的研究技术与方法,研究4周中等强度有氧运动对大鼠心房肌蛋白质组差异表达的影响,揭示中等强度有氧运动对心房肌物质能量代谢影响的规律。 方法:建立速度24m/min、持续时间40min(负荷强度相当于60%—70%VO2max),持续训练4周的中等强度有氧运动实验动物模型。应用双向凝胶电泳技术(2-DE)分离心房肌蛋白质点,串联飞行时间质谱仪技术鉴定电泳结果中表达量上调≥5倍以上,下调至1/5以下的13个备选目标蛋白质点。 结果:鉴定结果有7个与心房肌细胞物质能量代谢相关的蛋白或酶,其中线粒体乌头酸水合酶上调5.7倍,丙酮酸脱氢酶E1α1表达量上调6.4倍, 线粒体琥珀酸脱氢酶黄素蛋白亚基下调6.9倍,甲基丙二酸半醛脱氢酶[酰基]上调 6.3倍,异戊酰辅酶A脱氢酶下调6.9倍,线粒体二氢硫辛酸脱氢酶下调5.7倍,细胞质苹果酸脱氢酶表达量下调7.3倍。 结论:①中等强度有氧运动诱导心房肌蛋白质组表达量与强度出现显著变化,同时诱发心脏出现生理性肥大。②筛选出甲基丙二酸半醛脱氢酶[酰基],该蛋白在运动生物医学领域尚未展开深入研究,为运动改善心房肌物质能量代谢的研究带来新的研究内容。③中等强度有氧运动可能改善心房肌物质氧化代谢水平,上述差异蛋白是否具有运动依赖性和蛋白之间的联系还需进一步验证。
关键词:蛋白质组  有氧运动  心肌能量代谢  质谱鉴定  双向凝胶电泳
The influence of moderate aerobic exercise on the differential expression of energy metabolic protein in rat atrial muscle by based on proteomics research    Download Fulltext
Dept. of Health Sports Science, Nanjing Sport Institute, Nanjing, Jiangsu Province, 210014
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Abstract:
      Abstract Objective:To examine the effect of the 4 weeks of moderate intensity aerobic exercise on the differential expression among rat atrial muscle proteome and screen out the target protein spots in atrial muscle which are closely related to the aerobic exercise adaptation, and the effects of moderate intensity aerobic exercise on the metabolism of substance and energy in atrial muscle were revealed Method: We established a experimental animal model with 24m/min×40min sports load intensity training, which lasted for four weeks of aerobic exercise(load intensity equivalent to 60%—70%VO2max). 6 hours after the last training, we extracted the total protein from atrial tissue and the protein was separated by two-dimensional gel electrophoresis(2-DE). 13 target protein spots with more than 5 times increase or decrease to less than 1/5 will be identified by tandem time-of-flight mass spectrometer . Result: The identification results revealed 7 proteins or enzymes that related with atrial muscle cell energy metabolism: mitochondrial aconitate hydratase with 5.7 times increase, pyruvate dehydrogenase E1α1 with 6.4 times increase, mitochondrial succinate dehydrogenase flavoprotein subunit with 6.9 times decrease, methylmalonic semialdehyde dehydrogenase [acyl] up with 6.3 times increase, ISO isoamyl acyl coenzyme A dehydrogenase with 6.9 times decrease, mitochondrial dihydrolipoate acid dehydrogenase with 5.7 times decrease, cytoplasmic malate dehydrogenase expression with 7.3 times decrease. Conclusion: ①The moderate intensity aerobic exercise induced a significant change in the expression and intensity of atrial muscle proteome, while induced physiological hypertrophy in the heart.②It was found that the methyl malonate semialdehyde dehydrogenase [acyl] has not been involved in the sports medicine research. Intensive research for this protein will bring new research contents.③Moderate intensity aerobic exercise can improve the level of oxidative substance metabolism in atrial muscle. It is necessary to verify the exercise dependency and the connection between the difference protein in the future.
Keywords:proteomics  aerobic exercise  myocardial energy metabolism  mass spectrometry  two-dimensional gel electrophoresis
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