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尤莉蓉,赵 艳,夏 志.高强度间歇游泳训练对衰老小鼠骨骼肌蛋白质合成的影响研究[J].中国康复医学杂志,2019,(1):27~33
高强度间歇游泳训练对衰老小鼠骨骼肌蛋白质合成的影响研究    点此下载全文
尤莉蓉  赵 艳  夏 志
长江大学体育学院,荆州,434023
基金项目:江西省教育厅科技计划项目(GJJ160738)
DOI:
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摘要:
      摘要 目的:探讨高强度间歇训练对衰老小鼠快缩骨骼肌蛋白质合成的影响。 方法:16月龄雄性C57BL/6小鼠,20只据体重随机纳入对照组(C组)与高强度间歇运动干预组(CE组)。CE组小鼠每周一至五晚七点进行高强度间歇训练,为期8周。训练时小鼠在尾根绑缚相当于10%体重的负荷,游泳20s后休息10s,重复4组。每周增加1组训练,第8周时每次训练12组。实验前及训练后第2、4、6、8周的周日采用抓握力计测定肌力。末次训练24h后处死全部小鼠,同位素示踪法测定腓肠肌蛋白质合成率,Bradford法测定总蛋白、肌原纤维与肌浆蛋白浓度,Western blotting法检测mTORSer2448、4E-BP1Thr37/46与p70S6KThr389磷酸化率及MHCⅡ蛋白表达。 结果:8周高强度间歇训练导致腓肠肌蛋白质合成率、肌原纤维、肌浆蛋白质含量及蛋白质总量增加,mTORSer2448、p70S6KThr389及4E-BP1Thr37/46磷酸化率与MHCⅡ蛋白表达均升高,后肢抓握力亦有增长,且变化均具有显著性意义(P=0.000)。 结论:高强度间歇训练能够显著促进衰老小鼠腓肠肌骨骼肌蛋白质合成,并最终导致肌力增长,具有干预衰老性肌肉萎缩的潜力,其作用机理涉及骨骼肌内mTOR-p70S6K/4E-BP1的活化。
关键词:高强度间歇训练  衰老  骨骼肌  蛋白质合成
Effects of high-intensity interval swimming exercise training on protein synthesis in aging skeletal muscle of aged mice    Download Fulltext
School of Physical Education Yangtze University, Jingzhou, Hubei Province, 434023
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Abstract:
      Abstract Objective: To explore the protective effect and mechanism of regularly high-intensity interval training on protein synthesis in fast-twitch skeletal muscle of aging mice. Method: Twenty sixteen-month-old male C57/BL mice were randomly divided into control group and high-intensity interval training group by body weight. Mice in training group received 8 wks incremental swimming exercise training from Monday to Friday. These CE group mice repeated 4 sets of 20 seconds swimming at 10 seconds intervals with a workload of 10% body weight attached to the tails. The training sets were increased by 1set per wk, with the last wk having 12 sets. The grip strength was measured pre-study, and on Sunday of the second, fourth, sixth and eighth week post-intervention by using grip strength meter. Twenty four hours post the final exercise bout, mice were all sacrificed. The protein synthesis rate was measured using isotopic tracing. The total protein, myofibrillar protein and sarcoplasmic protein concentration in gastrocnemius muscles were detected by commercial Bradford protein assay kit. The phosphorylation rate of mTORSer2448, p70S6KThr389, 4E-BP1Thr37/46 and the protein expression of MHCⅡwere determined by using the Western blotting. The muscle strength was measured by the grip strength meter. Result: When compared with mice in control group, 8 wks high-intensity interval training increased the protein synthesis rate, the total, myofibrillar and sarcoplasmic protein content (P=0.000), and elevated the phosphorylation rate of mTORSer2448, p70S6KThr389, 4E-BP1Thr37/46, the protein expression of MHCⅡ and the hind limb grip strength with statistical significance (P=0.000). Conclusion: High-intensity interval training significantly promotes protein synthesis in gastrocnemius muscles of aging mice and finally leads to the elevated muscle strength. The mTOR-p70S6K/4E-BP1 pathway contributes to this effect. It's reasonable to believe that high-intensity interval training has the potential to treat the sarcopenia.
Keywords:high-intensity interval training  aging  skeletal muscle  protein synthesis
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