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张爱民,周增华,蒋宗滨.脉冲射频对坐骨神经分支损伤模型大鼠脊髓神经元凋亡的影响[J].中国康复医学杂志,2018,(10):1156~1161
脉冲射频对坐骨神经分支损伤模型大鼠脊髓神经元凋亡的影响    点此下载全文
张爱民  周增华  蒋宗滨
广西医科大学第二附属医院疼痛科,广西医科大学疼痛医学中心,南宁市大学东路,530007
基金项目:广西自然科学基金项目(2013GXNSFAA019137;2016GXNSFAA380172)
DOI:
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摘要:
      摘要 目的:探讨脉冲射频(pulsed radiofrequency,PRF)对坐骨神经分支损伤(spared nerve injury,SNI)模型大鼠脊髓神经元凋亡的影响。 方法:将32只雄性SD大鼠随机分为4组(n=8):空白对照组(C组)、模型组(SNI组)、假治疗组(Sham组) 和治疗组(PRF组)。于制模前0天,制模后1、4、7、10天,PRF治疗后6、12、24、36、48天分别测定4组大鼠机械缩足阈值(pain mechanical withdrawal threshold,PMWT);在PRF治疗前0天、治疗48天后分别处死每组大鼠4只,提取L4—L6节段脊髓,采用蛋白印记(Western Blot)技术检测bcl-2、caspase-3表达情况,TUNEL法检测脊髓组织凋亡细胞数。 结果:①坐骨神经分支损伤术后,SNI组、Sham组和PRF组大鼠的PMWT较C组显著降低(P<0.01);②经PRF治疗后,PRF组大鼠的PMWT可显著提高(P<0.05);③随着PMWT提高,PRF组bcl-2蛋白表达量显著增加,caspase-3蛋白表达量显著降低(P<0.05),而脊髓组织细胞凋亡数量显著下降(P<0.05)。 结论:PRF可能通过抑制脊髓神经细胞凋亡对SNI模型大鼠产生良好的镇痛作用。
关键词:脉冲射频  SNI模型  脊髓  凋亡
Effects of pulsed radiofrequency on apoptosis of spinal cord neurons in rats with spared nerve injury    Download Fulltext
Department of Pain, the Second Affiliated Hospital of Guangxi Medical University, Center for Pain Medicine of Guangxi Medical University,Nanning, Guangxi,530007
Fund Project:
Abstract:
      Abstract Objective:To explore the effect of pulsed radiofrequency (PRF) for spinal cord neuron apoptosis in rat withspared nerve injury (SNI). Method:Thirty-two male SD rats were randomly divided into four groups, ①control group (CON), ②SNI model group (SNI), ③sham treatment group (sham),④PRF treatment group (PRF). Pain mechanical withdrawal threshold (PMWT) was assessed before establishment of SNI model, 1,4,7 and 10d after establishment of SNI model, and 6,12,24,36,48d after PRF treatment. Four rats in each group were euthanized before PRF treatment and 48 days after treatment to collect L4—L6 spinal cord. Western blotting was conducted to assess the expression of bcl-2 and caspase-3 protein, and TUNEL method was used for apoptosis rate of spinal cord. Result:①The PMWT of SNI, Sham and PRF group were significantly decreased compared with CON group after spared nerve injury (P<0.01). ②The PMWT of PRF group was obviously enhanced after PRF treatment (P<0.05). ③The expression of bcl-2 was significantly up-regulated with the increase of PMWT while the expression of caspase-3 protein decreased (P<0.05), and the apoptosis rate was simultaneous decreased (P<0.05). Conclusion:PRF may produce good analgesic effect on SNI model rats by inhibiting the apoptosis of spinal cord neurons.
Keywords:pulsed radiofrequency  SNI model  spinal cord  apoptosis
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