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甄巧霞,洪 毅,白金柱,张军卫,姜树东.交替极性的直流电场对完全性脊髓损伤大鼠运动功能恢复的作用[J].中国康复医学杂志,2014,29(8):707~713
交替极性的直流电场对完全性脊髓损伤大鼠运动功能恢复的作用    点此下载全文
甄巧霞  洪 毅  白金柱  张军卫  姜树东
北京康复医院神经疾病康复中心,北京,100144
基金项目:北京市科技新星计划资助项目(2007B070)
DOI:
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摘要:
      摘要 目的:探讨交替极性的直流电场对大鼠完全性脊髓损伤运动功能恢复的作用。 方法:将30只成年SD雌性大鼠用改良Allen法(10g×2.5cm)制备完全性脊髓损伤模型后随机分为两组。两组均在损伤节段的上下各一个节段椎板间隙,放置交替极性的直流电场刺激器(可提供500—600μV/mm的直流电场,每15min改变一次极性),实验组实验后即刻给予直流电场环境干预至实验后2周,而对照组不予直流电场的条件。在实验后第1,3,7天,2周通过BBB评分观察双侧后肢运动功能的变化,并于实验后2周通过:①形态学:HE染色,免疫组织化学染色(神经丝蛋白、胶质纤维酸性蛋白)观察局部脊髓的变化。②神经电生理:运动诱发电位观察脊髓运动传导的变化。 结果:实验组和对照组实验后2周的BBB评分:实验组左后肢4.0±3.27,右后肢3.9±3.41;对照组左后肢4.9±3.35, 右后肢4.1±3.47;运动诱发电位潜伏期差:实验组左侧1.01±1.33,右侧0.16±0.09;对照组左侧2.15±4.44,右侧1.10±0.50;波幅差:实验组左侧1.86±1.45,右侧1.50±0.75;对照组左侧3.94±2.35,右侧2.76±1.30; 右下肢潜伏期差异有显著性意义(P<0.05),即脊髓运动传导有所改善。 结论:伤后2周,交替极性的直流电场能改善急性脊髓损伤大鼠的脊髓运动传导功能,但大鼠的运动功能恢复无显著性差异,组织学观察也未见显著性差异。直流电场对大鼠脊髓损伤的长期作用需进一步研究。
关键词:脊髓损伤  致伤装置  交替极性的直流电场  恢复
Function of stimulation of direct current field with alternative polarity on motor function recovery of rats with complete spinal cord injury    Download Fulltext
Beijing Rehabilitation Hospital,100144
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Abstract:
      Abstract Objective: To evaluate the function of stimulation of direct current field with alternative polarity on motor function recovery of rats with complete spinal cord injury. Method: Thirty female adult SD rats were contused by the modified Allen impact device to produce comple spinal cord injury(SCI) models and then were randomly assigned to two groups. The direct current field stimutelation devices (providing the alternative polarity stimulation of 500—600μV/mm, with polarity reversion every 15min) were placed one laminar segment above and below the injury area in both groups. The experimental group was stimulated after operation for 2 weeks, while the sham group had no stimulation. At the 1st d, 3rd d, 7th d, 2nd week after operation, Basso-Beattie-Bresnahan(BBB) scale was used to evaluate the neurological recovery. H-E staining, immunohistochemistry staining (for neurofilament and glial fibrillary acidic protein) and motor evoked potential(MEP) were used to evaluate the local changes and conductive changes of spinal cord at 2 weeks after operation. Result: The BBB scale after 2 weeks of the experimental group: the left 4.0±3.27,the right 3.9±3.41; the sham group:the left 4.9±3.35,the right 4.1±3.47; The latency difference of the MEP after 2 weeks of the experimental group: the left 1.01±1.33,the right 0.16±0.09,the sham group: the left 2.15±4.44,the right 1.10±0.50; The amplitude difference of the MEP after 2 weeks of the experimental group: the left 1.86±1.45,the right 1.50±0.75;the sham group: the left 3.94±2.35,the right 2.76±1.30.The latency difference of right hind leg in experimental group was shorter than that in sham group(P<0.05), it implied the spinal cord conduction improved. Conclusion: The stimulation of direct current field with alternative polarity could improve the spinal cord conduction of rats, but there was no significant difference in histological changes and neurological function between the two groups; and after the stimulation the injured rats' spinal cord can produce improvement in spinal cord conduction. However, the long-term effect of motor function recovery of direct current field in rats with spinal cord injury needs further investigation.
Keywords:spinal cord injury  impact device  direct current field with alternative polarity  recovery
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