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周治来,陈银海,靳安民,刘 巍.运动训练对脊髓损伤大鼠Nogo-A、NgR mRNA表达的影响[J].中国康复医学杂志,2011,26(2):143~147
运动训练对脊髓损伤大鼠Nogo-A、NgR mRNA表达的影响    点此下载全文
周治来  陈银海  靳安民  刘 巍
南方医科大学珠江医院骨科中心,广州,510282
基金项目:广东省自然科学基金项目(8451051501000460)
DOI:
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摘要:
      摘要 目的:探讨运动训练对脊髓损伤(SCI)大鼠髓鞘源性神经抑制因子(Nogo-A)及其受体(NgR)mRNA表达的影响。 方法:44只SD大鼠随机分为运动训练组16只、损伤组16只及假手术组12只,采用改良Allens打击法制作大鼠T10脊髓损伤模型。造模后每周应用BBB评分法观察大鼠后肢运动功能, 在第8、10、14天处死大鼠,每组4只,以荧光定量PCR法测定不同时间点脊髓组织中Nogo-A与NgR mRNA相对含量。 结果:手术后第4—8周,运动训练组大鼠BBB评分明显高于SCI组;运动训练组与SCI组Nogo-A与NgR mRNA表达明显高于假手术组(P<0.05),随着时间推移,运动训练组与SCI组Nogo-A与NgR mRNA表达量趋于下降。术后第8、10天,SCI组Nogo-A mRNA表达均显著高于运动训练组与假手术组(P<0.05),至第14天,运动训练组与SCI组表达差异无显著性意义(P>0.05),但均高于假手术组(P<0.05),假手术组在各时间点表达差异均无显著性意义(P>0.05);SCI组在各时间点NgR mRNA表达均高于运动训练组与假手术组(P<0.05),运动训练组在第10天便下降至与假手术组差异无显著性意义(P>0.05),假手术组在各时间点表达无差异(P>0.05)。 结论:康复训练能减少Nogo-A、NgR mRNA的表达,促进脊髓损伤大鼠后肢功能的恢复。
关键词:脊髓损伤  康复训练  Nogo-A  NgR  BBB评分
Effects of exercise training on the expressions of Nogo-A、NgR mRNA in spinal cord injury rats    Download Fulltext
Dept. of Orthopedic,Zhujiang Hospital,Southern Medical University,Guangzhou,510282
Fund Project:
Abstract:
      Abstract Objective:To explore the effects of exercise training on the expression of Nogo-A and its receptor NgR in spinal cord injury(SCI) rats. Method: Forty-four Sprague-Dawley rats were assigned into two groups(test group 32 rats and sham-operated group 12 rats)randomly. Rats of test group experienced modified Allen impactor(H=25mm)at T10, then were randomly divided into 2 groups equally: training-treated group and SCI group. The hindlimbs movement was assessed by Basso-Beattie-Bresnahan(BBB) scale and the injured spinal cord tissue was extracted for Nogo-A and NgR mRNA examination through SYBR Green real-time PCR on the 8th,10th,14th day after SCI. Result:Compared with SCI group,the BBB score in exercise training-treated group showed marked improvement between the 4th week and the 8th week after injury(P<0.05),Compared with sham-operated group,the expressions of Nogo-A and NgR mRNA in training-treated group and SCI group increased significantly postoperation(P<0.05),then declined gradually as time prolonged. The expression of Nogo-A mRNA in SCI group was significantly higher than that in training-treated group and sham-operated group on the 8th and 10th day postoperation(P<0.05), there was no significant difference between SCI group and training-treated group on the 14th day, but all higher than that in sham-operated group at the three time points, there was no statistical difference among the three time points in sham-operated group. The expression NgR mRNA in SCI group was significant higher than that in the two other groups at three time points; otherwise, that in training-treated group decreased almost the same as that in sham-operated group on the 10th day postoperation(P>0.05). There was also no statistical difference among the three time points in sham-operated group. Conclusion: Exercise training could down-regulate the expressions of Nogo-A and NgR mRNA in SCI rats effcectively,and promote functional recovery of hindlimbs.
Keywords:spinal cord injury  rehabilitation training  Nogo-A  NgR  BBB score
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