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吴 岚,刘开祥,俸军林,曾爱源,李 浩.青藤碱对大鼠脑缺血再灌注损伤环氧化酶-2表达及前列腺素E2含量的影响[J].中国康复医学杂志,2009,24(4):293~296
青藤碱对大鼠脑缺血再灌注损伤环氧化酶-2表达及前列腺素E2含量的影响    点此下载全文
吴 岚  刘开祥  俸军林  曾爱源  李 浩
桂林医学院附属医院神经内科,广西,桂林,541002
基金项目:广西自然科学基金资助(桂科自0848015);广西卫生厅科研课题基金资助(Z2008262)
DOI:
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摘要:
      目的:探讨青藤碱(sinomenine,Sin)对缺血再灌注损伤大鼠脑组织的保护作用及对环氧化酶-2 (cyclooxygenase-2,COX-2) 表达和前列腺素E2(prostaglandin E2,PGE2)含量的影响。方法: 将大鼠随机分为假手术组、缺血再灌注组、Sin低剂量治疗组和Sin高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。Sin低剂量(30mg/kg)、高剂量(60mg/kg)治疗组于术前30min分别给予大鼠腹腔注射。用免疫组化法观察缺血90min再灌注24h大鼠额顶部皮质COX-2表达,放免法检测PGE2含量,检测脑含水量变化,并进行2,3,5-三苯基氯化四氮唑(tetrazolium chloride,TTC)染色和HE染色观察脑梗死体积及病理形态学变化。结果: ①Sin高、低剂量治疗组脑含水量、脑梗死体积较缺血再灌注组减小,Sin高、低剂量组之间差异亦具有显著性意义(P<0.05)。②Sin高、低剂量治疗组脑组织缺血损伤病理学改变明显轻于缺血再灌注组,Sin高剂量治疗组缺血改变亦轻于低剂量治疗组。③与假手术组比较,缺血再灌注组额顶部皮质COX-2表达明显增加;与缺血再灌注组比较,Sin高、低剂量治疗组COX-2表达均显著减少(P<0.05);高、低剂量组之间差异亦具有显著性意义(P<0.05)。④与假手术组比较,缺血再灌注组额顶部皮质PGE2含量明显升高;与缺血再灌注组比较,Sin高、低剂量治疗组PGE2含量明显降低(P<0.05),高、低剂量组之间差异有显著性意义(P<0.05)。结论: Sin对缺血再灌注脑损伤具有保护作用,其机制可能与通过抑制再灌注损伤后COX-2表达,减少PGE2含量有关。
关键词:缺血再灌注  环氧化酶-2  前列腺素E2  青藤碱
Effects of sinomenine on expressions of cyclooxygenase-2 and prostaglandin E2 content following cerebral ischemic reperfusion injury in rats    Download Fulltext
Dept. of Neurology,the Affiliated Hospital of Guilin Medical College,Guilin,541002
Fund Project:
Abstract:
      Objective:To study the effects of sinomenine(Sin) on expressions of cyclooxygenase-2(COX-2) and prostaglandin E2(PGE2) content following cerebral ischemia reperfusion(I/R) injury in rats. Method:Rats were randomly divided into 4 groups:sham operated group, I/R group,low dose Sin(30mg/kg) treated group and high dose Sin(60mg/kg) treated group. The focal middle cerebral artery occlusion(MCAO) model was made by suture-occlusion method. Sin were given intraperitoneally 30min before focal cerebral ischemia operation respectively. After MCAO 90min and following 24h of reperfusion, expressions of COX-2 using immunohistochemistry and PGE2 content in frontal and parietal cortex were investigated. Water content of Brain was measured with TTC and HE staining.Result:①Compared with that of I/R group, in low and high dose Sin treated group, water content of Brain and cerebral infarction volume reduced dose-dependently(P<0.05). ②The change of ischemic impairment in low or high dose Sin treated group was less than that in I/R group,and in high dose Sin treated group that was even lesser than that in low dose group. ③Compared with sham operated group,in I/R group the expressions of COX-2 in frontal and parietal cortex increased at 24h of reperfusion(P<0.05). Compared with I/R group,in low and high dose Sin treated group the expression of COX-2 reduced dose-dependence(P<0.05). ④Compared with sham operated group, in I/R group PGE2 content in frontal and parietal cortex increased at 24h of reperfusion(P<0.05). Compared with I/R group, in low and high dose Sin treated group PGE2 content was less dose-dependent(P<0.05). Conclusion:Sinomenine may obviously reduce cerebral infarct volume,reduce cerebral ischemia-reperfusion injure by decreasing the expressions of COX-2 and PGE2 content.Sinomenine plays protective effect on cerebral ischemia injury.
Keywords:ischemia-reperfusion  cyclooxygenase-2  prostaglandin E2  sinomenine
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