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郑玲玲,吴建中,支 晔,韦培湧,薛雯娣.臂丛神经损伤对热休克蛋白和神经型一氧化氮合成酶表达的影响[J].中国康复医学杂志,2008,23(8):729~731
臂丛神经损伤对热休克蛋白和神经型一氧化氮合成酶表达的影响    点此下载全文
郑玲玲  吴建中  支 晔  韦培湧  薛雯娣
汕头大学医学院解剖学教研室,汕头,515041
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摘要:
      目的:观察臂丛神经远侧不同损伤后脊髓前角运动神经元中热休克蛋白70(Hsp70)、热休克蛋白27(Hsp27),以及神经型一氧化氮合成酶(nNOS)的表达变化,探讨其与运动神经元存活和再生的相互关系。方法:选用18只健康成年雄性SD大鼠,随机分为3组:肌皮神经切断组、挫伤组和对照组。术后2个月进行Grooming实验,检测其患肢运动功能的恢复。后取脊髓C5节段标本,观察脊髓运动神经元中三种蛋白的表达情况。结果:神经切断组和挫伤组的运动功能恢复均较明显,4级以上的百分比挫伤组(86%)优于切断组(57%)。与对照组相比,两实验组损伤侧脊髓运动神经元中三种蛋白的表达均显著上调。切断组较挫伤组表达较高水平的Hsp70(140.61±1.25,130.08±2.38)和Hsp27(143.86±1.32,138.93±1.58);而nNOS蛋白在挫伤组中有较高水平的表达(挫伤组:106.12±0.91;切断组:68.18±0.63)。结论:肌皮神经切断和挫伤后脊髓运动神经元中三种蛋白表达有所不同,其与损伤后功能恢复直接相关。
关键词:肌皮神经  热休克蛋白  神经型一氧化氮合成酶  运动神经元
The impact of brachial plexus injury on the expressions of heat shock proteins and neuronal nitric oxide synthase    Download Fulltext
Dept. of Anatomy, Medical College of Shantou University, Shantou, 515041
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Abstract:
      Objective: To observe the expression changes of Hsp70, Hsp27 and nNOS in spinal motoneurons following different distal injury of brachial plexus and to investigate the correlation of the expression changes and the survival and regeneration of motoneurons. Method: A total of eighteen adult male SD rats were randomly divided into three groups: musculocutaneous nerve cut group(n=7), musculocutaneous nerve crushed group(n=7) and control group(n=4). Two months later, motor function was assessed by Grooming test standard to evaluate the functional recovery at the experimental side. Then the C5 spinal cord were harvested and immunohistochemical examination were performed. Result: In cut group and crushed group, the function recovery were all significant. Moreover, the percentage of above grading 4 in crushed group(86%) was better than in cut group(57%). Compared to control group, the expressions of all the three proteins at motoneurons of experimental side in the two experimental groups increased significantly. The number of Hsp70 and Hsp27 positive motoneurons in cut group(140.61±1.25,130.08±2.38) were more than that in crushed group(143.86±1.32,138.93±1.58), but the number of nNOS positive motoneurons in crushed group(106.12±0.91) was more than that in cut group(68.18±0.63). Conclusion: The expressions of three proteins in spinal motoneurons following musculocutaneous nerve cut and crush lesion were different from the normal, and it were directly correlated with the motor function recovery.
Keywords:musculocutaneous nerve  heat shock protein  neuronal nitric oxide synthase  motoneuron
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